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Proposal of an HPLC/UV/FLD Screening Method for the Simultaneous Determination of Ten Antibiotics in Environmental Waters

Idalia Francisca Carmona-Alvarado, Maria de la Luz Salazar-Cavazos, Noemi Waksman de Torres, Aurora de Jesús Garza-Juarez, Lidia Naccha Torres, Jose Francisco Islas, Norma Cavazos-Rocha

Abstract


An HPLC-UV/FLD method for simultaneous detection of ten antibiotics in surface waters was developed. Antibiotics were extracted from water using solid phase extraction. An Atlantis T3 column was used with acetonitrile and 0.05% trifluoroacetic acid as a mobile phase for separation, with a total running time of 45 min. Signal detection was performed at 280 nm; fluoroquinolones were additionally quantified by fluorescence detection. Validation parameters such as linearity, recovery and precision were evaluated. The limits of detection (LOD) in river waters were in the range 0.1-1.3 µg/L for antibiotics detected by UV, and 0.039 and 0.073 µg/Lfor fluoroquinolones detected by FLD. LOD are sufficiently low to consider this method as a first alternative for HPLC-MS methods that will allow alerting for the presence of antibiotics in surface waters. This screening method is rapid, sensitive, reproducible and economical.


Keywords


Antibiotic analysis; emergent contaminants; simultaneous analysis by HPLC-UV/FLD; fluoroquinolones and sulfonamides; multiresidue analysis method; superficial water analysis.

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DOI: http://dx.doi.org/10.17344/acsi.2021.6870

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Copyright (c) 2020 Idalia Carmona-Alvarado, Maria de la Luz Salazar-Cavazos, Noemi Waksman de Torres, Aurora Garza-Juarez, Lidia Naccha Torres, JOSE FRANCISCO ISLAS, Norma Cavazos

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This work is licensed under a Creative Commons Attribution 4.0 International License.