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Determination of Microelements in Human Milk and Infant Formula Without Digestion by ICP-OES

Dijana Đurović, Branka Milisavljević, Mirjana Nedović-Vuković, Branislav Potkonjak, Snežana Spasić, Miroslav Vrvić

Abstract


The concentrations of  zinc (Zn), iron (Fe) and copper (Cu) in both human milk and infant formula were determined using a new sample preparation method, by inductively coupled plasma – optical emission spectometry (ICP-OES) and flame atomic absorption spectrometry (FAAS). Human milk samples were diluted in ultrapure water. The infant formula of powder samples (suitable for an infant 1-6 months of age) and standard reference material (SRM-1849) were analyzed in parallel.

The results have shown that FAAS method was more sensitive for Fe determination in human milk while ICP-OES was more sensitive for both Zn and Cu detection. The limit of quantification   for both Zn and Cu was 5 µg L-1 and 10 µg L-1 for Fe and the recovery for Zn, Fe and Cu was ranged from 90 % to 94 %, 97 % to 103 % and 90% to 102 %, respectively. Mean concentrations of Zn, Fe, and Cu in human milk samples were 5.35, 0.47 and 0.83 mg L-1, respectively while these values in infant formula were ranged from 3.52 - 4.75 mg L-1, 3.37 - 4.56mg L-1 and 0.28 - 0.41 mg L-1, respectively.

Despite the sample complexity, the proposed method using dilution of milk samples with waterwas simple, rapid, effective and accurate. ICP-OES was a better method for Zn determination while FAAS was a better method for Fe determination. In the case of Cu both methods were comparable.


Keywords


Microelements; human milk; method validation; no sample preparation

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DOI: http://dx.doi.org/10.17344/acsi.2016.2582

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Copyright (c) 2017 Dijana Đurović, Branka Milisavljević, Mirjana Nedović-Vuković, Branislav Potkonjak, Snežana Spasić, Miroslav Vrvić

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